化学発光免疫測定法（ChLIA)

Chemiluminescence immunoassays (ChLIA) from EUROIMMUN use magnetic particles coated with antibodies or antigens as a solid phase to detect specific antigens or antibodies in patient samples by means of chemiluminescence signals. Performance and evaluation are fully automated with the IDS-iSYS or the IDS-i10.

Antibody detection by ChLIA

The antigen-coated magnetic particles are incubated with diluted patient samples. If a sample contains specific antibodies directed against the antigen, these bind to the antigen-coated magnetic particles. In the next step, acridinium-labelled antibody (conjugate) is added. This binds to the specific antibodies.  A trigger solution is then added to induce a chemiluminescence reaction. The resulting light signal is proportional to the antibody concentration in the patient sample within the measurement range and is automatically converted into a concentration by the instrument.

Antigen detection by ChLIA

The antibody-coated magnetic particles are incubated with the patient sample and an antigen-specific biotinylated antibody. During the incubation, the antigen is bound by the magnetic particle-coupled antibody as well as by the biotinylated antibody. In the next step, acridinium-labelled ExtrAvidin (conjugate) is added. This binds to the biotinylated antibody. A trigger solution is then added to induce a chemiluminescence reaction. The resulting light signal is proportional to the antigen concentration in the patient sample within the indicated measurement range and is converted automatically into a concentration by the instrument.